Cleanascite™ Employed to Investigate Lipid Influence in Tumor-associated macrophages (TAMs)

MONMOUTH JUNCTION, N.J. - Jan. 16, 2023 - PRLog -- Biotech Support Group reports on an article, describing the simplicity and efficiency of their lipid clearance technology for depletion of lipids from omental conditioned medium (OCM) to determine their influence on tumor-associated macrophages (TAMs)

Chan, David, et al. "Polyunsaturated Fatty Acids Promote Protumoral Macrophage Polarization via a RhoA-YAP1 Signaling Pathway in the Ovarian Cancer Microenvironment (https://assets.researchsquare.com/files/rs-2384228/v1/512f673f-2190-4a52-bda6-21b2f6ddbbb7.pdf?c=1672353575)." (2022).

This study reports that polyunsaturated fatty acids (PUFAs) in malignant ascites promote protumoral M2-like TAMs deposition and facilitate peritoneal metastases of epithelial ovarian cancer (EOC). The article states "… the removal of free fatty acids in OCM by the Cleanascite™ attenuated lipid droplets deposition … indicating protumoral M2-like TAMs exhibited higher lipid accumulation and metabolism in the fatty acid-enriched omental conditioned medium (OCM) or the malignant ascites." "… Similarly, …we further found …removal of lipids by Cleanascite™ remarkably prevented the reduction of YAP1 in OCM-Macrophages.." The article concludes that PUFAs are a key player in promoting tumor-infiltrated TAMs polarization that, in turn, facilitates EOC tumor growth and metastasis.

"This is now our fifth study whereby Cleanascite™ was used in evaluating the removal of free fatty acids in omental conditioned medium. These references along with over twenty others, show that Cleanascite™ can help identify a characteristic feature of in vitro cell response related to lipid removal. Unlike alternative methods that use solvents, Cleanascite™ is an aqueous suspension product and so it is very compatible with cellular models of disease. This ultimately helps with investigations such as this, that need to determine whether or not lipids, or factors associated with lipids, impart phenotypic changes to cells." states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.

Link to view our case study, Lipid Removal for Phenotypic Cell Response in Cancer Research (biotechsupportgroup.com) (https://www.biotechsupportgroup.com/category-s/324.htm)

For more information visit: Cleanascite™ Lipid Removal Reagent and Clarification, at
http://www.biotechsupportgroup.com/Cleanascite-Lipid-Removal-Reagent-p/x2555.htm

Keywords: Tumor-associated macrophages (TAMs), Macrophage polarization, omental conditioned medium, Cleanascite™, polyunsaturated fatty acids (PUFAs), malignant ascites

For Business Development, contact: Matthew Kuruc 732-274-2866, mkuruc@biotechsupportgroup.com (mailto:sales@biotechsupportgroup.com)