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Follow on Google News | AnaSpec Introduces Ultra-Sensitive SensoLyte® NADP/NADPH Assay KitSensoLyte® Fluorimetric NADP/NADPH Assay Kit is a sensitive fluorimetric assay that detects NADP and NADPH without interfering with NAD/NADPH.
By: AnaSpec EGT Group Pyridine nucleotides are involved in a number of critical catabolic and anabolic reactions in living organisms. Nicotinamide adenine dinucleotide phosphate (NADP) is a natural coenzyme present in all animal and plant organisms. Cellular NADPH is important for tolerance to ROS and maintenance of cellular redox homeostasis.1- In addition to the fluorimetric kit, AnaSpec also provides a convenient colorimetric kit. This kit also detects NADP and NADPH without interfering with NAD/NADH. The SensoLyte® Colorimetric NADP/NADPH Assay Kit utilizes an enzyme cycling reaction to produce a blue colored product, formazan, which can be monitored at 565 nm using a microplate reader. The intensity of the color produced is proportional to NADP/NADPH concentration. References 1. Holmgren, A. J. Biol. Chem. 264, 13963 (1989). 2. Grant, CM. et al. Mol. Microbiol 21, 171 (1996a). 3. Muller, EG. et al. Mol. Biol.Cell 7, 1805 (1996). 4. Ng, CH. et al. Free Radic. Biol. Med. 44, 1131 (2008). 5. Temple, MD. et al. Trends Cell Biol. 15, 319 (2005). 6. Stamler, JS. et al. Science 258, 1898 (1992). 7. Kirkman, HN. et al. J. Clin. Invest. 55, 875 (1975). 8. Magnani, M. Acta Haematol. 75, 211 (1986). For more information, please visit http://www.anaspec.com/ # # # AnaSpec, Inc. is a leading provider of integrated proteomics solutions™ for worldwide life science research. AnaSpec offers expertise in three primary technologies: End
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