Tips and Tricks for Proper Pipetting

April 23, 2012 - PRLog -- Pipettes are used frequently in laboratories and results are dependent on their reliable performance. Are you sure your pipette is dispensing the correct amount? While here are regular pipette checkups that can ensure for functioning and accurate pipettes,did you know that your handling also has a great influence on pipette-performance?

Proper pipetting technique directly affects the success and repeatability of your experiments. Below are a few simple things to remember that will greatly improve your pipetting performance.

• Immersion depth
Immersing the tip too far can cause more liquid to be aspirated. Conversely, if the tip is not immersed far enough, air can be aspirated. Both result in inaccurate volume. Correct tip immersion depth can improve accuracy by up to 5%, so use the recommended depths shown here. (>2000 μl, use 6-10 mm depth)

• Consistent pipette rhythm and speed
Maintainingconsistent pipetting rhythm and speed will produce optimal and more repeatable results. Fast or “jerky” aspiration can cause splashing, aerosols, shaft contamination and loss of sample volume. With consistent rhythm and speed you can achieve up to 5% accuracy improvement.

• Constant temperature
Theideal temperature for pipetting is at 21.5degree C±1 degree C-thesame used for calibration. Avoid drafty or sunlit areas that could cause the large or sudden temperature changes that compromise aspiration accuracy. Pipetting at a constant temperature can improve results by as much as 5%.

• Vertical immersion angle
Keep the immersion angle vertical or within 20 degree of vertical-angles greater than that can produce inaccurate measurements. For micro volume pipettes, keeping the angle close to vertical can improve accuracy by up to 2.%.


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