pCREB Transcription Factor Activity Assay Kit (Colorimetric)

MILPITAS, Calif. - Oct. 18, 2021 - PRLog -- CREB (cAMP response element-binding protein) is a phosphorylation-dependent cellular transcription factor. It is activated by phosphorylation by various serine-threonine kinases such as PKA (cAMP-dependent protein kinase A), PKC (protein kinase C) and CaMKs (calmodulin kinases) on the serine 133 residue.

Once activated, pCREB interacts with the coactivator proteins and binds to the cAMP response elements (CRE) in the promoter region at 5' upstream region and increases or decreases the transcription of genes including c-fos, BDNF, tyrosine hydroxylase, numerous neuropeptides etc.

CREB is involved in regulating a variety of cellular processes, including cell survival, cell proliferation, glucose homeostasis, spermatogenesis, circadian rhythm, synaptic plasticity associated with memory etc.

BioVision's pCREB Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of pCREB in nuclear extracts or cell lysates. The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the CRE is coated on the 96-well plate.

Active pCREB in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of pCREB primary antibody that recognizes the pCREB-CRE comlex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.

Figures: Transcription factor activity assay using nuclear extracts of HEK 293 cells. Assay was performed following the assay kit protocol.

Storage Conditions Multiple Temperatures
USAGE For Research Use Only! Not For Use in Humans.
For more information on this Assay Kit Cat# K2109 https://bit.ly/3DRPClj