Albumin depletion using Albusorb™ For Proteomic Profiling of Cerebrospinal Fluid

Dec. 2, 2012 - PRLog -- MONMOUTH JUNCTION, NJ.– Authors Pottiez et al published a chapter in the book Expression Profiling in Neuroscience, Neuromethods titled, Proteomic Profiling of Cerebrospinal Fluid, on proteomic profiling platforms which analyze cerebrospinal fluid (CSF) for protein biomarkers and developing protein profiles of CSF for early identification of neurological diseases. Authors provide examples of affinity-based systems for removing most abundant proteins and cite Albusorb™ albumin depletion kit from Biotech Support Group. Moreover variations of protein concentration yielded by immunodepletion of CSF samples from nondemented (ND) patients and patients with HIV-associated dementia (HAD) are recorded. For detailed protocols click
http://link.springer.com/protocol/10.1007%2F978-1-61779-448-3_13

Albusorb™ is an albumin depletion reagent supplied as a kit with necessary buffers. AlbuSorb™ comes from a silica-based, separation platform utilizing a new combination of surface microenvironments substituted with low molecular substrates that feature drug-binding motifs. Unlike immuno-affinity, the surfaces utilized are disposable eliminating cycle to cycle variance and cross-contamination.

Characteristics of Albusorb™

Removes >90% albumin from 30 mg albumin/ml sample
Affinity-type equivalence, virtually no cross-reactivity with other proteins
Bind and elute procedure - simply weigh powder, condition the sample, centrifuge and/or filter, and recover the albumin depleted serum
Economical new surface technology, not based on affinity chromatography
Mild conditions maintain tertiary structure of proteins and simple transfer to secondary analysis
The albumin depleted filtrate retains the enzymatic and biological activity
Removes albumin from samples such as serum, plasma and from species including human, mouse, sheep, bovine, goat, rat, and calf
The flow through fraction is compatible with LC-MS, activity based protein profiling and proteomic studies.

For more information about AlbuSorb™, click:
http://www.biotechsupportgroup.com/node/64

AlbuVoid™ - Albumin Depletion Kit from Serum or Plasma
http://www.biotechsupportgroup.com/node/55

Biotech Support Group also has AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment.

About Biotech Support Group LLC
Biotech Support Group LLC is a leading provider of genomics and proteomics sample preparation products and enrichment reagent kits as well as integrated biotechnology services for life sciences research, biomarker and drug discovery. Based in New Jersey, it’s principal products include:  Albusorb™ for albumin depletion, AlbuVoid™ for albumin depletion plus low abundance serum protein enrichment, Cleanascite™ for lipid adsorption and clarification, NuGel™ for passivated silica-based affinity chromatography, and ProCipitate™ & ProPrep™ for nucleic acid isolation. Biotech Support Group is the leading developer of sample preparation products for separating and purifying hemoglobin from blood samples. HemoVoid™ is a hemoglobin depletion reagent kit from red blood cells and HemogloBind™ is a hemoglobin capture reagent from hemolyzed serum. Currently, Biotech Support Group LLC and ProFACT Proteomics Inc., are collaborating on the development of a proteomics platform used in functional profiling for proteomic analysis and a separations method for generating sub-proteomes used in biomarker and functional proteomic prospecting. For more information, go to: www.biotechsupportgroup.com  

CONTACT:
Ravish Amin
Biotech Support Group
1 Deer Park Drive, Suite M,
Monmouth Junction, NJ 08852, USA          
732-274-2866
sales@biotechsupportgroup.com

AlbuSorb™ References

Rat Serum

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e83b82098d4e9798d154c3ad738c83

Cerebrospinal Fluid

Gwenael Pottiez, Pawel Ciborowski. Proteomic Profiling of Cerebrospinal Fluid Expression Profiling In Neuroscience Neuromethods.2012;64:245-270
http://www.springerlink.com/content/j4u06x2k73u11476/#section=1013451&page=1

Serum

Happonen KE, Fürst CM, Saxne T et al. PRELP protein inhibits the formation of the complement membrane attack complex.Journal of Biological Chemistry.2012;287(11):8092-100
http://www.ncbi.nlm.nih.gov/pubmed/22267731

Holmberg R, Refai E, Höög A.Lowering apolipoprotein CIII delays onset of type 1 diabetes. Proceedings of the National Academy of Sciences.2011;108(26):10685-9.
http://www.pnas.org/content/early/2011/06/08/1019553108.abstract?elq=06e83b82098d4e9798d154c3ad738c83

Tang MX, Ogawa K, Asamoto M. Effects of Nobiletin on PhIP-Induced Prostate and Colon Carcinogenesis in F344 Rats  Nutrition and Cancer.2011;63(2):227-33
http://www.researchgate.net/publication/49814854_Effects_of_Nobiletin_on_PhIP-Induced_Prostate_and_Colon_Carcinogenesis_in_F344_Rats

Holmberg, Rebecka Apolipoprotein CIII and Ljungan virus in diabetes 2010. Doctoral Thesis
http://publications.ki.se/jspui/handle/10616/39507

Lu Q, Zheng X, McIntosh T Development of different analysis platforms with LC-MS for pharmacokinetic studies of protein drugs. Analytical Chemistry.2009;81(21):8715-23
http://www.ncbi.nlm.nih.gov/pubmed/19788250

Suggested References

Kabat, Elvin A., Murray Glusman, and Vesta Knaub. "Quantitative estimation of the albumin and gamma globulin in normal and pathologic cerebrospinal fluid by immunochemical methods." The American journal of medicine 4.5 (1948): 653-662.
http://www.sciencedirect.com/science/article/pii/S0002934348903891

Cutler, R. W. P., G. V. Watters, and J. P. Hammerstad. "The origin and turnover rates of cerebrospinal fluid albumin and gamma-globulin in man."Journal of the neurological sciences 10.3 (1970): 259-268.
http://www.sciencedirect.com/science/article/pii/0022510X70901541

Ganrot, Karin, and Carl-Bertil Laurell. "Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation." Clinical chemistry 20.5 (1974): 571-573.
http://www.clinchem.org/content/20/5/571.short

Habgood, M. D., et al. "A developmentally regulated blood-cerebrospinal fluid transfer mechanism for albumin in immature rats." The Journal of physiology456.1 (1992): 181-192.
http://jp.physoc.org/content/456/1/181.short

Müller, Norbert, and Manfred Ackenheil. "Immunoglobulin and albumin content of cerebrospinal fluid in schizophrenic patients: relationship to negative symptomatology." Schizophrenia research 14.3 (1995): 223-228.
http://www.sciencedirect.com/science/article/pii/092099649400045A

Stevens, R. W., et al. "Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin." Journal of Clinical Microbiology 10.3 (1979): 346-350.
http://jcm.asm.org/content/10/3/346.short

Cutler, Robert WP, Ruthmary K. Deuel, and Charles F. Barlow. "Albumin exchange between plasma and cerebrospinal fluid." Archives of Neurology 17.3 (1967): 261.
http://archneur.ama-assn.org/cgi/reprint/17/3/261.pdf