MONMOUTH JUNCTION, N.J. -
Sept. 23, 2015 -
PRLog -- MONMOUTH JUNCTION, NJ -- Biotech Support Group reports on a recent research article describing the simplicity and efficiency of their proteomic sample preparation technology for clearing detergent from perfused heart effluents.
The citation is:
Reyes, Levy A., et al. "Depletion of NADP (H) due to CD38 activation triggers endothelial dysfunction in the postischemic heart." Proceedings of the National Academy of Sciences (2015). http://www.pnas.org/
cgi/doi/10.1073/
pnas.1505556112
In brief, the article’s authors aimed at studying causes of postischemic endothelial dysfunction and possible new therapeutic targets for prevention of unstable coronary syndromes. The article states “Detection of Pyridine Nucleotides Following Endothelial Permeablization…
Before HPLC analysis…Triton X-100 was removed from samples with addition of SurfactAway™
(BioTech Support Group) in a 1:4 dilution. Samples were concentrated using a SpeedVac (Thermo Scientific) and resuspended in 250 μL of potassium cyanide (KCN) buffer (KCN 0.2 M, KOH 0.06 M, and DTPA 1 mM). SurfactAway™
was again added at 1:4 dilution to ensure complete removal of Triton X-100, concentrated, and then HPLC measurements were performed…”
“This is an excellent example of how
SurfactAway™ can improve HPLC measurements, in this case for the analysis of Pyridine Nucleotides. There was a need to remove spectral interferences without compromising the quantity or quality of the analytes present. So the selectivity profile of
SurfactAway™ makes metabolite profiling very easy.” states Swapan Roy, Ph.D., President and Founder of Biotech Support Group.
For more information visit: SurfactAway™
Triton Removal, at
http://biotechsupportgroup.com/surfactaway-triton-removalAbout Biotech Support Group LLCBiotech Support Group LLC is a leading provider of proteomic, metabolomic and genomic sample preparation and enrichment products and services. It’s principal products include: AlbuVoid™ & AlbuSorb™ for albumin depletion, Cleanascite™
for lipid adsorption and clarification, HemogloBind™
& HemoVoid™ for hemoglobin removal, NuGel™ for functional & chemical proteomics, and ProCipitate™
for nucleic acid isolation. For more information, go to http://www.biotechsupportgroup.com
Contact:Dr. Swapan Roy & Matthew Kuruc
Biotech Support Group LLC
1 Deer Park Drive, Suite M
Monmouth Junction NJ 08852
732-274-2866 Worldwide
800-935-0628 North America
sales@biotechsupportgroup.comhttp://www.biotechsupportgroup.com
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