Importance Of Endotoxin Testing In Hemodialysis Procedure

March 25, 2012 - PRLog -- Endotoxin: A pyrogenic substance (lipopolysaccharide) produced by gram-negative bacteria. Endotoxins can cause fever, rigors, hypo tension, organ failure and even death if they get into the circulation.

There is mounting evidence to suggest that exposure to relatively low levels of endotoxin causes chronic inflammation and there is increasing evidence linking inflammation to cardiovascular disease in the general public but the effect of inflammation on rates of mortality and morbidity in haemodialysis patients is still under debate. To be certain that the health of patients is not being compromised, dialysis units should aim to minimise their exposure to any detectable level of endotoxin.


Water used for haemodialysis should be tested routinely to verify the efficacy of the disinfection programme, Disinfection is a less lethal process than sterilization, since it is expected to destroy most recognized pathogenic microorganisms, but not
necessarily all those present. Disinfection does not eliminate endotoxin,  it is stable, it is not easily destroyed by sanitizers , Only ultrafiltration  removes endotoxin.  

The LAL assay is the method recommended for endotoxin testing by the most Pharmacopoeias, and is widely used  the pharmaceutical industry.

All the established techniques (gel-clot, turbidimetric and chromogenic) for LAL testing can be used for testing water for haemodialysis.

The LAL gel-clot test and Chromogenic Endpoint assay can be perfomed very  easily in  a dialysis unit. The suppliers of the LAL reagent will normally provide training in the testing and validation procedures.

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