BioVision Introduces Industry’s First Cholesterol Efflux Assay Kit (cell-based)!

MILPITAS, Calif. - Jan. 31, 2014 - PRLog -- Cholesterol Efflux Fluorometric Assay Kit (cell-based) (Catalog # K582-100) - 100 assays: Numerous studies have established a negative correlation between cellular cholesterol efflux and atherosclerosis. Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. Standard protocol for quantitation of cholesterol efflux involves labelling cells with tritiated cholesterol & measuring the release of labeled sterol. However, this protocol is not ideal for high-throughput screening of large numbers of serum samples or screening compounds/small molecules that affect cholesterol efflux.

Key Features and Benefits of the Kit:
- Simple & Rapid Protocol
- Convenient: Non-Radioactive, no special handling or disposal required
- High-Throughput
- Useful for screening serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux or screening small molecules that influence cholesterol efflux (a valuable tool for drug discovery program)
- Extremely Stable: long shelf life
- Accurate: reproducible results with low intra & inter assay variability
- Fluorometric Quantitation (Ex/Em = 482/515 nm)
- Ample reagents to perform 100 assays in 96-well plate format

For more information, please visit http://www.biovision.com/cholesterol-efflux-fluorometric-assay-kit-cell-based-7966.html