HMSiR – Fluorescent Probe for Live-Cell Super-Resolution Imaging

GÖTTINGEN, Germany - Aug. 5, 2016 - PRLog -- Due to the diffraction of light, the resolution of conventional light microscopy is limited. Super-resolution techniques allow images to be taken with a higher resolution than the diffraction limit providing many new insights in cellular biology. Direct stochastic optical reconstruction microscopy (dSTORM) is a super-resolution imaging technique that utilizes sequential activation and time-resolved localization of photoswitchable fluorophores to create high-resolution images, allowing spatial resolution of about 20 nm.

The new released HMSiR series displays spontaneous blinking when it emits fluorescence. Unlike the emission of other probes - which show a constant fluorescence signal - this probe is flashing. Until now fluorophores did not blink by itself without the addition of additives. On the other hand, however, additives are interfering with live cell imaging. By using the HMSiR series super-resolution imaging is now possible without additives like thiols or oxygen scavengers. Furthermore, irradiation with high-power laser was necessary in previous dSTORM observations. This new probe enables live-cell super-resolution imaging with low-power excitation light retaining physiological conditions.

Features

• Imaging possible under physiological conditions

• HMSiR displays spontaneous blinking with low-power excitation light and without additives

• Overcoming the problems of dSTORM

• Performing super-resolution imaging without thiols or oxygen scavengers

• No high-power laser irradiation necessary

• Minimize cell damage

For details please see: http://www.mobitec.com/cms/products/bio/07_fluores_tec/HM...

GORYO Chemical is a Japan-based company spezialized in creating new fluorescent probes for bio applications. Further activites include custom synthesis of fluorescent probes and the development of diagnostic reagents with fluorescent probes.

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.