Actin polymerization using K457
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April 10, 2018 -
PRLog -- Actin (G- and F-actin) is often referred as the family of fibrillary proteins that can be found in almost all eukaryotes. In general, the formation of filamentous F-actin occurs when an Actin-ATP undergoes hydrolysis releasing inorganic phosphate (Pi) and ADP-Actin. F-actin is then formed by reversible, non-covalent processes using ADP-Actin molecules. Actin plays critical roles in cell mobility and cell contraction during cell division among others processes. BioVision is excited to introduce Actin Polymerization/
Depolymerization Assay Kit. The assay utilizes proprietary pyrene-labeled actin that generates a stable signal if actin undergoes polymerization. Our assay is simple, high-throughput compatible, and can be used to study the effect of diverse potential anti-polymerization agents.
Key Features:
• Non-radioactive, versatile assays (Microplate)
• Specific, homogeneous assay
• Sensitive: fluorometric format
• Convenient: minimal sample preparation, fast protocols (< 3 hours)
• Cost effective: 100 assays; High Throughput Screening (HTS) compatible
• Adaptable: works with most of the commonly available instrumentations
Figures. A. Actin Polymerizarion using K457: Actin Polymerization is chemically induced (red). Actin polymerization is inhibited by Latrunculin A* (green). B. Actin Depolymerization:
Polymerized Actin (red) is depolymerized by Latrunculin A (green). Assays were performed following the kit protocols. *Note: Latrunculin A is Actin polymerization inhibitor in vitro and in vivo by the formation of a 1:1 complex with monomeric G-actin. Latrunculin A acts a depolymerization agent acting on Actin filaments (F-actin).
To get more information on this product visit:
https://www.biovision.com/actin-polymerization-depolymeri...Visit www.BioVision.com
