PicoProbe™ Glutaminase (GLS) Activity Assay Kit (Fluorometric)

MILPITAS, Calif. - March 15, 2019 - PRLog -- Glutaminase (EC 3.5.1.2) is a mitochondrial enzyme that hydrolyzes glutamine producing glutamate and ammonia. GLS is one of the two key enzymes that are responsible for glutamine homeostasis, the other being glutamine synthetase. Since glutamine is a major metabolic substrate involved in gluconeogenesis, its homeostasis is tightly controlled. Glutaminase has tissue-specific roles in multiple organs, with the isoform GLS1 being expressed in kidneys and brain, and GLS2 being expressed in the liver. It is involved in maintenance of acid-base
homeostasis by producing ammonia during renal acidosis in kidneys; and regulation of glutamate –which acts as a neurotransmitter- in the brain. On the other hand, GLS 2 regulates the generation of ammonia in liver by producing urea. Research shows GLS increased activity in individuals facing starvation, diabetes and high protein diets.
BioVision's PicoProbe^TM Glutaminase Activity Assay Kit is a simple platebased fluorometric assay for measuring glutaminase activity in biological samples. GLS hydrolyzes glutamine forming glutamate and ammonia. Glutamate, in the presence of a developer and enzyme mix converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 2 µU of glutaminase in biological samples.

Figures:(a) Glutamate standard curve (b) Enzyme kinetics for rat brain lysate (3.9 µg protein per well) and rat liver lysate (9.5 µg protein per well) (c) Glutaminase specific activity in rat brain (GLS1) and liver tissue (GLS2). Experiments were conducted according to K455-100 kit protocol.

For more information on assay, visit: https://www.biovision.com/picoprobetm-glutaminase-gls-act...