Aldose Reductase Activity Kit (Colorimetric)

MILPITAS, Calif. - May 13, 2019 - PRLog -- Aldose Reductase (Aldehyde Reductase; AR; ALR2; EC 1.1.1.21), a member of the aldo-keto reductase superfamily, catalyzes the NADPH-dependent reduction of a wide variety of aldoses (molecules containing carbonyl groups) to their corresponding alcohols. It is a key enzyme in the polyol (polyhydric alcohol) pathway and has been implicated to play a critical role in diabetes and cardiovascular complications. Aldose Reductase is the key factor in the reduction of glucose to sorbitol and its activity is higher during hyperglycemic
conditions. The synthesis and accumulation of sorbitol due to AR activity is the main cause of diabetic complications, such as cataracts, retinopathy, neuropathy and nephropathy. Thus, AR could be used as a therapeutic target in the clinical treatment of secondary complications of diabetes.
BioVision's Aldose Reductase Activity Kit is based on the ability of AR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The assay is simple, sensitive and can detect as little as 0.1 mU of Aldose Reductase activity in a variety of sample types.
Figure: Aldose Reductase specific activity was calculated from Rat Kidney (20 µg protein) or HepG2 cell lysates (110 µg protein). Assays were performed following the Kit-369  protocol.

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