MILPITAS, Calif. -
Nov. 25, 2019 -
PRLog -- Proline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honey bees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 µmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones. BioVision's PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.
Figure: Estimation of proline in local honey, human serum, spinach leaves lysate and rat liver lysate. Proline concentrations were 425.3 ± 48.9 mg/kg in local honey, 248.2 ± 3.90 µM in human serum, 0.877 ± 0.015 µmol/g-wet wt in spinach leaves and 13.3 ± 1.4 pmol/µg-protein in rat liver lysate. Assays were performed following the kit K2020 protocol.
For more information on this kit, visit:
https://www.biovision.com/picoprobetm-proline-assay-kit-fluorometric.htmlVisit: www.biovision.com
