CaspGLOW™ Green Active Caspase-3/7 Staining Kit

MILPITAS, Calif. - Jan. 15, 2020 - PRLog -- Activation of caspases plays a central role in apoptosis. The CaspGLOW^TM Active Caspase-3/7 Staining Kit provides a convenient means for detecting activated caspase3/7 in living cells. The assay utilizes the caspase inhibitor DEVD-FMK conjugated to FITC (Green-DEVD-FMK) as the fluorescent in situ marker. Green-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to the activated caspase-3/7 in apoptotic cells. The green fluorescence label allows the direct detection of activated caspase-3/7 in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader. The caspase inhibitor, Z-VAD-FMK, is included in the kit as an additional control. It can be used to inhibit caspase activation in an induced culture.

For Flow Cytometric analysis, resuspend cells in 300 µl of Wash buffer. Put samples on ice. Analyze samples by Flow Cytometry using the FL-1 channel.
For Fluorescence Microscopic analysis, resuspend cells in 100 µl Wash buffer. Put one drop of the cell suspension onto a microslide and cover with a coverslip. Observe cells under a fluorescence microscope using FITC filter. Caspase positive cells appear to have brighter green signals whereas caspase negative control cells show a much weaker signal.
For analysis with Fluorescence Plate Reader, resuspend cells in 100 µl Wash Buffer and then transfer the cell suspension to each well of the black microtiter plate. Measure the fluorescence intensity at Ex/Em = 488/530 nm. For control, use wells containing unlabeled cells.

Figure: Sample Flow Cytometry Histogram for Camptothecin treated Jurkat Cells:
For more information on this assay kit, visit: https://www.biovision.com/caspglowtm-green-active-caspase-3-7-staining-kit-23946.html