Production of nano antibodies by immunizing alpaca-KMD bioscience provides you with quality services

TIANJIN, China - Nov. 10, 2023 - PRLog -- Nanobodies (Nbs), a new type of antibody, first discovered in camel blood by Belgian scientist Hamers et al. Compared with traditional antibodies, Nanobody, whose molecular weight is only about 15 KDa, does not have a light chain, only a heavy chain antibody (HcAb) and two conventional CH2 and CH3 regions.Therefore, it is called a nanoantibody and is the smallest unit known to bind to a target antigen.

Production method of nano antibody

Because of these characteristics of nano-antibodies, more and more research institutions and drug manufacturers pay attention to and try to use nano-antibodies in different scenarios. The development of nano antibodies is different from the traditional monoclonal antibody Production method by hybridoma, which generally selects candidate nano antibodies by immunizing alpaca, constructing phage library and displaying phage, and then carries out the verification experiment of binding to antigen after expression and purification of nano antibodies.

Phage display technology (i.e., nano antibody technology) is a biotechnology that inserts the DNA sequence of a foreign protein or polypeptide into the appropriate position of the structure gene of the phage coat protein, usually the P3 protein of the M13 phage, so that the foreign gene is displayed on the surface of the phage with the expression of the coat protein. The advantage of phage display is that it displays the diversity of mutant antibody genes in the nanoantibody library, and the surface of each recombinant phage (commonly used phage is M13 phage) displays different antigen-binding domains. Compared with the traditional hybridoma method, Phage display technology has a very prominent efficiency advantage in the level of antibody gene display and screening (such as hybridoma fusion efficiency is general < 0.4%, which is about 4 fusion cells out of 1,000 fusion cells; Phage display can easily obtain 10⁸-10⁹ correct antibody sequences).

KMD Bioscience has established a complete and mature phage antibody display technology platform. Based on the phage display technology platform, KMD Bioscience can provide major experimental links including antigen design, alpaca immunity, library construction and screening, and activity function verification, and provide highly specific and high-affinity alpaca VHH antibodies to scientists around the world. In addition, KMD Bioscience has rich experience in antibody engineering construction, and can provide three-dimensional antibody upstream and downstream services, including antibody humanization service, human scFv antibody library construction service, human Fab antibody library construction service, human antibody phage library Production service, etc.
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