Transcreener® Assays From BellBrook Labs: Two Additional Plate Readers Certified

GÖTTINGEN, Germany - Dec. 1, 2016 - PRLog -- Transcreener® assays manufactured by BellBrook Labs, USA, distributed in Europe by MoBiTec GmbH, Germany, enable you to screen more targets, both well characterized and emerging, faster and more efficiently. Thousands of cellular reactions use nucleotides, either alone or in combination with other substrates. Enzymes in the purinome alone - those that use adenine and guanine nucleotides - comprise 13% of the coding capacity of the human genome. Of these, protein kinases have become highly validated drug targets, but lipid kinases, chaperone ATPases and GTPases as well as less obvious ATP-dependent enzymes such as Acetyl Co-A carboxylase are increasingly being targeted for therapeutic intervention. Direct detection of nucleotides is unique to the Transcreener® platform.

Increased instrumentation costs and limited budgets mean that most HTS labs rely on a single type of multimode plate reader, which may limit the detection modes that can be used. The ability of an assay technology to generate different types of readouts and perform well on a variety of plate readers is certainly an asset in this regard. Transcreener® assays are available in a three fluorescent detection modes.

1. Time Resolved–Förster Resonance Energy Transfer (TR-FRET)
2. Fluorescent Intensity (FI)
3. Fluorescent Polarization (FP)

This allows the user to not only pick a mode that is best for the study, but also one that can be found on a plate reader in his or her own lab. No additional equipment required.
Validation
As new multimode readers are introduced to the market, the three Transcreener® detection modes are validated individually to ensure full compatibility and define optimal settings.

Most recently, the Tristar² LB 942, and Mithras² LB 943 plate readers from Berthold Technologies were certified for use with BellBrook's Transcreener® FI and TR-FRET Assay platforms by an independent lab at the University of Bonn in Germany. The testing for FP is in final stages and the results will be available soon. Please us for an updated status.

BellBrook's Plate Reader Validation Criteria

• 384-Well Format
• Z'-Factor ≥ 0.7 at 10% conversion of 10 μM ATP or GDP
• Read time to achieve Z' specifications ≤ 5 minutes

Customers from Europe are welcome to visit www.mobitec.com  or contact MoBiTec GmbH for Transcreener® assay product pricing. Custom quotes are available for bulk orders.

About Bellbrook Labs

BellBrook Labs is dedicated to providing the pharmaceutical industry with enabling screening tools to accelerate the discovery of more effective therapies. As a small, R&D-driven company, BBL identifies areas where there is a strong need but a significant technical hurdle, and then BBL develops a solution. The company is focused on taking creative approaches to new product development and to continually improving the level of service provided to customers.

Transcreener® Biochemical Assay Technology

BellBrook's proprietary Transcreener® HTS Platform (https://www.bellbrooklabs.com/transcreener-hts-assays/) was developed to move more new targets into HTS more rapidly, and to streamline selectivity profiling within a target family. It relies on homogenous fluorescent immunodetection of nucleotides, including ADP, UDP, GDP, AMP, and GMP, that are formed by thousands of cellular enzymes. Among these are group transferase families, such as kinases and glycosyltransferases, that catalyze the covalent regulatory reactions that are central to cell signaling. Each Transcreener Assay provides a generic detection method for multiple enzyme families. This means that scientists can focus on evaluating new therapeutic strategies and optimizing lead molecules rather than on time-consuming assay development for individual targets. Since the introduction of the first Transcreener assays in 2005, the platform has been rapidly validated and adopted by pharmaceutical and biotechnology screening labs around the world.

About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company (founded in 1987) that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics and proteomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.