MILPITAS, Calif. -
Feb. 21, 2020 -
PRLog -- Sucrose Phosphorylase (EC 2.4.1.7) is a transglucosidase enzyme, which plays a key role in the metabolism of sucrose, starch as well as other metabolites. It catalyzes the conversion of sucrose to α-D-glucose-
1-phosphate (G1P) and D-fructose. It is present in both gram positive and negative bacteria such as in Leuconostoc mesenteroides, Bifidobacterium longum, Pseudomonas sacclwrophila and Lactobacillus reuteri. Sucrose Phosphorylase is used as a biocatalyst to produce fructose syrup in food industry and for determining inorganic phosphate in clinical analysis. BioVision's Sucrose Phosphorylase Activity Assay Kit can be used to determine the activity of Sucrose Phosphorylase in different microorganisms. The assay converts Sucrose to G1P, which is then detected by a set of enzymatic reactions to generate a colored product. The colored product can be measured by absorbance (OD 450 nm) and is directly proportional to the Sucrose Phosphorylase activity. BioVision's Sucrose Phosphorylase Activity Assay Kit is a rapid, sensitive and a convenient tool for measuring Sucrose Phosphorylase activity. It can detect as low as 10 mU under the assay conditions.
Figures: A. G1P Standard Curve. B. Reaction curve of the Sucrose Phosphorylase activity. C. Sucrose Phosphorylase activity in bacterial lysates before and after spiking with 10 mU of the Sucrose Phosphorylase. Assay was performed according to the kit K2034 protocol.
For more information on this assay kit, visit:
https://www.biovision.com/sucrose-phosphorylase-activity-assay-kit-colorimetric.html
