CRISPR/Cas9 - Request a FREE Sample for Your Genome Editing Research

GÖTTINGEN, Germany - Sept. 8, 2016 - PRLog -- Bacteria and archaea exhibit chromosomal elements called clustered regularly interspaced short palindromic repeats (CRISPR) that are part of an adaptive immune system that protects against invading viral and plasmid DNA. In Type II CRISPR systems, CRISPR RNAs (crRNAs) function with trans-activating crRNA (tracrRNA) and CRISPR-associated (Cas) proteins to introduce double-stranded breaks in target DNA. Target cleavage by Cas9 requires base pairing between the crRNA and tracrRNA as well as base pairing between the crRNA and the target DNA (see figure CRISPR/Cas9 Genome Editing). Target recognition is facilitated by the presence of a short sequence called a protospacer-adjacent motif (PAM) that conforms to the sequence NGG.

The bacterial CRISPR/Cas9 system has been adapted to serve as a versatile platform for RNA-directed genome editing in mammalian cells. The Cas9 endonuclease can be programmed by a dual RNA (crRNA and tracrRNA), or the core components of these RNAs can also be combined into a single hybrid guide RNA. Once Cas9 has cleaved the target DNA, two endogenous repair mechanisms, non-homologous end joining (NHEJ) and homology-directed repair (HDR), are triggered in response to the DNA break. The features of these DNA break repair pathways can be exploited to generate gene knock-outs or introduce defined modifications at the site of cleavage. NHEJ is an error-prone process that frequently results in the formation of small insertions and deletions that disrupt gene function. HDR requires homologous DNA as a template for repair and can be leveraged to create a limitless variety of modifications specified by the introduction of donor DNA containing the desired sequence flanked on either side by sequences bearing homology to the target.

The simplicity of using a noncoding RNA guide to target DNA for site-specific cleavage provides a distinct advantage over alternative genome editing technologies such as ZFNs and TALENs. Using the CRISPR/Cas9 strategy, retargeting the nuclease complex only requires introduction of a new RNA sequence and there is no need to reengineer the specificity of DNA-binding proteins.

Request a FREE Sample for Your Genome Editing Research

TransIT-X2^® Dynamic Delivery System

• A novel, polymeric system for delivery of multiple nucleic acids to mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

• DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
• Guide RNA – Deliver guide RNA oligonucleotides targeting your gene of interest
• RNP – Deliver Cas9/guide RNA ribonucleoprotein complexes

TransIT^®-mRNA Transfection Kit

• A high-efficiency, low toxicity transfection reagent for mammalian cells. Delivers CRISPR/Cas9 components in the following formats:

• mRNA – Deliver messenger RNA expressing Cas9
• Guide RNA – Deliver guide RNA targeting your gene of interest

Ingenio^® Electroporation Solution

• A high-efficiency electroporation solution compatible with most conventional electroporation devices, including Lonza-Amaxa^®, Bio-Rad^®, or Harvard BTX^®. Delivers CRISPR/Cas9 components in the following formats:

• DNA – Deliver plasmid DNA expressing Cas9 or guide RNA
• mRNA– Deliver messenger RNA expressing Cas9
• Guide RNA– Deliver guide RNA oligonucleotides targeting your gene of interest
• RNP– Deliver Cas9/guide RNA ribonucleoprotein complexes

Visit Us for More Information:

• http://www.mobitec.com/cms/products/bio/02_genomics/crisp... (http://www.mobitec.com/cms/products/bio/06_cell_bio/trans...)

Prove It to Yourself!

• * Sample Policy: MoBiTec together with Mirus considers sample requests for up to three different transfection products and one electroporation product per laboratory IN GERMANY (please contact us if from a different country). Please include all samples to be tested in a single request and mail to: info@mobitec.com


About MoBiTec GmbH

MoBiTec GmbH (Goettingen, Germany) is a privately held company, founded in 1987, that offers research tools for molecular and cell biology. Products include DNA vectors for cloning and expression, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, products for genomics, proteomics, lipidomics, and glycomics research, numerous antibodies and recombinant proteins, superior fluorescence reagents and kits, affinity chromatography products, as well as general laboratory equipment.

In parallel to its own product lines, MoBiTec distributes products from international companies in Germany. MoBiTec products are distributed worldwide, in Germany from their home office, in other countries by distributors.