MILPITAS, Calif. -
April 13, 2020 -
PRLog -- Alpha-Mannosidase (α-Man; EC 3.2.1.24) is a glycoside hydrolase involved in the cleavage of the alpha-mannose. α-Man can be applied in studies that determine the effects of mannose on specific molecules, such as recombinant proteins that are used for vaccine development. Lysosomal α-Man is essential for catabolizing the Asn-linked glycans of glycoproteins and is actively involved in maintaining the cellular homeostasis. α-Man deficiency results in Alpha-Mannosidosis, an acquired lysosomal storage disorder in which α-Man fails to break down mannose-containing oligosaccharides. These oligosaccharides, therefore, accumulate in lysosomes thereby leading to cellular malfunction and apoptosis. BioVision's Alpha-Mannosidase Activity Assay Kit provides a facile, rapid way to monitor total αMan activity in various biological samples. In this kit, α-Man cleaves a synthetic specific substrate and releases a fluorophore, which can be easily quantified (Ex/Em = 360/445 nm). The substrate is specific to α-Man and can differentiate from β-mannosidase. The assay is simple, sensitive and can detect as low as 1.0 µU of alpha mannosidase activity.
Figures: (a). 4-Methylumbelliferone (4-MU) Standard Curve. (b). Measurement of purified α-Mannosidase (0.6 ng) and β-Mannosidase (43 ng) activities using our proprietary substrate. The kit can efficiently distinguish α-Mannosidase activity from β-Mannosidase. (c). αMannosidase activity in human plasma (10 μl), 3T3 cells (2 μg protein), and rat liver (10 μg protein). All assays were performed following the kit protocol.
For more information on this assay kit, please visit:
https://www.biovision.com/alpha-mannosidase-activity-assay-kit-fluorometric.html
